**Human Laminin (LN) ELISA Kit – Handling and Usage Instructions**
The Human Laminin ELISA Kit should be stored at 2–8°C and allowed to equilibrate at room temperature for 20 minutes before use. If the concentrated washing solution is taken out from the refrigerator, it may crystallize, which is a normal occurrence. To dissolve the crystals completely, warm the solution in a water bath before use.
Unused microtiter strips should be immediately returned to the ziplock bag and resealed to maintain a low-temperature, dry storage environment. This helps preserve the integrity of the kit components for future use.
When handling samples, no dilution is required if the sample has been pre-treated. Simply add 10 μL of the prepared sample directly into the wells. It is crucial to follow the incubation times, volumes, and procedural sequence as specified in the protocol to ensure accurate results. Any deviation may affect the sensitivity and specificity of the assay.
Before using any liquid reagents, make sure to shake them thoroughly to ensure proper mixing and homogeneity. This step is essential for maintaining consistency across all experimental runs.
Additionally, always wear appropriate personal protective equipment (PPE), such as gloves and lab coats, when handling the reagents. Avoid cross-contamination by using separate pipette tips for each sample and reagent. Proper waste disposal should also be followed according to local laboratory guidelines.
By following these detailed instructions, you can maximize the performance and reliability of the Human Laminin ELISA Kit.
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