Are you looking for an answer to these human ELISA kit experiments?
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July 30 08:27:06, 2025
At our company, we place the highest priority on product quality and strictly adhere to contractual obligations. We maintain rigorous standards for both delivery timelines and experimental techniques, which has led to an increasing number of satisfied customers. Since the establishment of Shanghai Hengyuan five years ago, we have gathered and summarized various challenges faced by teachers and researchers, helping many solve their experimental issues. Are you looking for solutions to common problems when using human ELISA kits?
**· Why is the standard curve not linear?**
Ensure that the standard is stored according to the manufacturer's instructions. Before dissolving the standard, centrifuge it thoroughly. Make sure the standard is fully dissolved and well-mixed before proceeding with serial dilutions. Each step should be carefully mixed and accurately pipetted. Also, ensure proper termination of the color development process. Choose an appropriate mathematical model to plot the standard curve for better accuracy.
**· Why should all reagents and samples be brought to room temperature before use?**
Temperature plays a crucial role in the ELISA reaction. To ensure consistent reactions across all samples, all reagents and test samples must be equilibrated to room temperature before starting the experiment. This helps avoid discrepancies caused by temperature differences during the reaction process.
**· Why should duplicates be set up in ELISA experiments?**
To achieve more accurate results, it is highly recommended to run both standards and samples in duplicate. Duplicate testing allows you to: calculate an average value for greater precision, identify and correct errors caused by operational mistakes, and assess the coefficient of variation (CV) to evaluate the reliability of your experiment and the kit performance.
**· What causes poor repeatability?**
This could be due to several factors:
1. Variations in sample volume or loading time. (Solution: Keep the loading time as consistent as possible when repeating samples.)
3. Incorrect microplate reader filter settings or wrong wavelength input. (Solution: Use 450/630 nm for TMB color development.)
4. Poor reproducibility from the microplate reader. (Solution: Hengyuan offers calibration services for microplate readers.)
Our human ELISA kits are designed to be safe, reliable, and easy to use. Available in two specifications—96T and 48T—our kits are compatible with high-quality reagent brands such as R&D Systems and TSZ from the United States. Whether you're conducting research or clinical testing, our products are engineered to deliver consistent and accurate results. Let us help you achieve better outcomes in your experiments.