Are you looking for an answer to these human ELISA kit experiments?
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July 30 08:32:48, 2025
At our company, we place a strong emphasis on product quality and strictly adhere to contractual obligations. We maintain high standards for both delivery timelines and experimental techniques, which has led to an increasing number of satisfied and successful clients. Since the establishment of Shanghai Hengyuan five years ago, we have continuously gathered and analyzed teachers' feedback, helping many researchers resolve their experimental challenges. If you're seeking solutions to your human ELISA kit experiments, here are some common issues and their explanations.
**Why is the standard curve not linear?**
To ensure accurate results, the standard should be stored according to the manufacturer's instructions. Before dissolving, it’s important to centrifuge the sample thoroughly. Make sure the standard is fully dissolved and mixed before proceeding with serial dilutions. Each step must be well mixed and pipetted accurately. Also, the color development should be terminated at the appropriate time. Choosing a suitable mathematical model for curve fitting will help improve the linearity of the standard curve.
**Why should all reagents and test samples be equilibrated to room temperature before use?**
Temperature plays a crucial role in the ELISA reaction. To ensure consistent results across all samples, all reagents—including test samples—should be brought to room temperature before starting the experiment. This helps avoid discrepancies caused by varying temperature responses, leading to more reliable outcomes.
**Why is it necessary to set up duplicates in human ELISA experiments?**
To achieve more accurate results, it is strongly recommended to run both standards and samples in duplicate. Duplicate testing allows for calculating an average value, reducing errors caused by random fluctuations. It also helps identify and correct any anomalies due to operational mistakes. Additionally, it enables the calculation of the coefficient of variation (CV), which reflects the precision of the experiment and the performance of the kit.
**Why is there poor repeatability in the experiment?**
Possible causes include:
1. Variations in the number of samples or inconsistent loading times. (Solution: Keep the loading time as consistent as possible when repeating samples.)
3. Incorrect microplate reader filter settings or wrong wavelength input. (Solution: Use TMB substrate with 450/630 nm wavelength.)
4. Poor reproducibility from the microplate reader. (Solution: Hengyuan offers calibration services for the reader.)
Today’s information is just the beginning. Our human ELISA kits are known for their safety, reliability, and high performance. Available in two specifications—96T and 48T—the kits use high-quality reagents from renowned brands such as R&D Systems and TSZ. Whether you’re conducting routine experiments or complex research, our products are designed to meet your needs with accuracy and consistency. Let us support your scientific progress with confidence.