Human IFN-α ELISA Kit
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September 26 00:13:28, 2025
**Human IFN-α ELISA Kit – For the Quantitative In Vitro Determination of Human Interferon α in Serum, Plasma, Cerebrospinal Fluid, Tissue Homogenate, and Other Biological Fluids**
*For Laboratory Research Use Only. Not for Diagnostic or Therapeutic Purposes.*
This kit is designed for the quantitative measurement of Human Interferon α (IFN-α) concentrations in various biological samples. The assay is based on a sandwich ELISA format using specific antibodies that recognize IFN-α. A standard curve is generated using known concentrations of IFN-α, and sample concentrations are determined by comparing their optical density (OD) values to this curve.
**Key Features:**
- **High Sensitivity:** Detects as low as 1.0 pg/mL.
- **Wide Dynamic Range:** 1.25 pg/mL to 40 pg/mL.
- **Excellent Specificity:** No significant cross-reactivity with other cytokines or interferons.
- **User-Friendly Protocol:** Simple and straightforward procedure suitable for routine laboratory use.
**Sample Collection and Preparation:**
- **Serum:** Collect using a serum separator tube. Allow clotting for 2 hours at room temperature or overnight at 4°C. Centrifuge at 2000×g for 20 minutes. Remove serum and assay immediately or store at -20°C. Avoid repeated freeze-thaw cycles.
- **Plasma:** Collect using heparin as an anticoagulant. Centrifuge within 30 minutes at 2000×g for 30 minutes at 2–8°C. Store at -20°C.
- **Cell Culture Supernatants, Tissue Homogenates, and Other Body Fluids:** Centrifuge to remove particulates. Assay immediately or store at -20°C. Ensure no hemolysis or granulation occurs.
**Materials Required (Not Supplied):**
- 37°C incubator
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes, disposable tips, and absorbent paper
- Distilled or deionized water
**Reagents Provided (Stored at 2–8°C):**
- Microtiter Strip Plate (12 x 8 strips / 12 x 4 strips)
- Standard (6 vials, 0.5 mL/vial)
- Sample Diluent (6.0 mL / 3.0 mL)
- HRP-Conjugate Reagent (10.0 mL / 5.0 mL)
- 20X Wash Solution (25 mL / 15 mL)
- Chromogen Solution A (6.0 mL / 3.0 mL)
- Chromogen Solution B (6.0 mL / 3.0 mL)
- Stop Solution (6.0 mL / 3.0 mL)
- Closure Plate Membrane (2 pieces)
- User Manual (1 copy)
- Sealed Bags (1 each)
**Important Notes:**
- Standard concentrations: 40, 20, 10, 5, 2.5, 1.25 pg/mL.
- If sample OD exceeds the highest standard, dilute with Sample Diluent and retest.
- Do not use reagents beyond expiration date.
- Allow all reagents and samples to reach room temperature (20–25°C) before use.
- Always use fresh pipette tips to prevent contamination.
- Dispose of all biological materials according to local biohazard regulations.
- Viruses must be inactivated before disposal (allow 30 minutes contact time).
**Assay Procedure:**
1. Prepare all reagents and microplate strips.
2. Add 50 µL of standards or samples to appropriate wells. Cover with adhesive strip and incubate for 60 minutes at 37°C.
3. Wash plate 4 times with 1X Wash Solution (manual or automated).
4. Add 50 µL of Chromogen A and 50 µL of Chromogen B to each well. Incubate for 15 minutes at 37°C, protected from light.
5. Add 50 µL of Stop Solution to each well. Read OD at 450 nm using a microplate reader.
6. Plot standard curve and calculate sample concentrations accordingly.
**Quality Control:**
- Intra-assay CV < 15%
- Inter-assay CV < 15%
- Storage: 2–8°C (for frequent use), -20°C (long-term storage, up to 6 months)
**Safety Information:**
- Handle all samples and reagents with care. Treat all biological materials as potentially infectious.
- Follow good laboratory practices and wear appropriate personal protective equipment (PPE).
**Storage Instructions:**
- Store unused strips in sealed bags with desiccant at 2–8°C.
- Do not expose reagents to heat or direct sunlight.
**Note:** This product is intended for research purposes only. It is not approved for diagnostic use. Always refer to the complete user manual for detailed instructions and safety information.