2. Regularly clean the solvent bottles and all related components to maintain a contamination-free environment. When changing the mobile phase, always check the solubility between the two solvents. If they are not fully miscible, use an intermediate solvent for transfer and thoroughly rinse the system to avoid precipitation or blockage.

3. After completing the experiment, make sure to flush the pump carefully with the appropriate solvent to prevent wear on the seals and reduce the risk of clogging. When working with buffer solutions, extra attention is needed, as salts can crystallize and cause damage if not properly flushed out.

4. Always flush the sample or buffer salt remaining in the injector before shutting down the system. This helps prevent cross-contamination between samples, inorganic salt buildup, and potential damage to the hardware or tubing due to blockage.

5. Keep the detector clean and free from debris. After each use, clean the detector with the column and use degassed mobile phase to avoid bubble formation inside the flow cell. If the detector is not in use for more than four hours, consider turning off the lamp to save energy and prolong its lifespan.

6. Ensure that the detector's cooling fan and vents remain unobstructed. Avoid making unnecessary changes to the inlet and outlet lines of the detector, as this can affect performance and lead to leaks or malfunctions.

7. The reference cell of a differential detector must be cleaned thoroughly before and after each use to ensure accurate readings and prevent contamination that could interfere with measurements.

8. When tightening the drain valve, apply moderate force to avoid over-tightening, which could damage the seal or cause leakage. Always check for any signs of leakage after installation.

By following these maintenance tips, you can significantly improve the reliability and longevity of your liquid chromatography system while ensuring consistent and accurate results in your experiments.

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