At 3 p.m. yesterday, Manager Shanghai Hengyuan received a call from Manager Li of the technical department. Manager Li mentioned: "After summarizing this month's customer inquiries about our ELISA kits, I noticed several key points that are often overlooked. I've compiled the essential information and will send it to your email. Please review it and share the insights with our customers — this can greatly improve their experimental outcomes." Below is the latest update from Manager Li of Shanghai Hengyuan Technology, based on the June Technical Summary. **Key Points to Consider When Using ELISA Kits:** 1. **Read the instructions carefully** and ensure the kit is within its expiration date before starting the experiment. 2. **Verify that all reagents and required equipment are present**, including test tubes, pipette tips, pipettes, centrifuges, and other necessary tools. 3. **Standardize specimen preparation** by preparing sufficient volumes (at least 2–3 replicates) for each sample. If the samples cannot be tested immediately, store them properly using cryopreservation techniques. 4. **Allow reagents to reach room temperature** as specified in the protocol before use to ensure accurate results. 5. **Choose appropriate antibodies**: It’s best to pair a monoclonal antibody with a polyclonal one. If using two monoclonal antibodies, ensure they recognize different epitopes. It’s also recommended to select antibody pairs from the same manufacturer for better compatibility. 6. **Optimize the standard curve** by performing a 2-fold serial dilution of the antigen standard, ranging from 2000 pg/ml down to 15 pg/ml. To enhance sensitivity, consider using standard ELISA protocols, amplification kits, third-party reagents, or modifying the enzyme substrate system. Shanghai Hengyuan updates its website weekly with the latest experimental knowledge and industry insights. If you're looking to purchase high-quality ELISA kits, feel free to contact our technical team at Shanghai Hengyuan Biotechnology Co., Ltd. We offer full technical support and even provide free testing services to help you achieve the best results. Let us assist you in improving your experiments today!

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